Lidstrom:Tube Spec: Difference between revisions
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==Tube Spec== | ==Tube Spec== | ||
* minimum volume: | * minimum volume: 5 mL | ||
* use green-capped test tubes (larger diameter) | * use green-capped test tubes (larger diameter) | ||
* use a blank containing your media | * use a blank containing your media | ||
* ok to leave on all day, but do turn it off when done | * ok to leave on all day, but do turn it off when done | ||
** bulbs are cheap (UV bulbs are generally the expensive ones) | ** bulbs are cheap (UV bulbs are generally the expensive ones) | ||
==To Use== | |||
# Turn on by turning leftmost dial clockwise | |||
# Set filter to 600 nm (or whatever) | |||
# Zero transmittance with left knob | |||
# Put in blank & cover with black cap | |||
# set transmittance to 100 | |||
# absorbance for the blank should now be 0 | |||
# Measure absorbance for your sample | |||
* Keep tube orientation the same each time you measure in case of any unevenness. | |||
[http://www.cienytec.com/PDFS/Espec_SPEC20_OpMan_ing.pdf manual] | [http://www.cienytec.com/PDFS/Espec_SPEC20_OpMan_ing.pdf manual] | ||
Revision as of 17:40, 7 June 2012
Return: Protocols
Tube Spec
- minimum volume: 5 mL
- use green-capped test tubes (larger diameter)
- use a blank containing your media
- ok to leave on all day, but do turn it off when done
- bulbs are cheap (UV bulbs are generally the expensive ones)
To Use
- Turn on by turning leftmost dial clockwise
- Set filter to 600 nm (or whatever)
- Zero transmittance with left knob
- Put in blank & cover with black cap
- set transmittance to 100
- absorbance for the blank should now be 0
- Measure absorbance for your sample
- Keep tube orientation the same each time you measure in case of any unevenness.
