Lidstrom:Tube Spec

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==Tube Spec==
==Tube Spec==
-
* minimum volume: 10 mL
+
* minimum volume: 5 mL
* use green-capped test tubes (larger diameter)
* use green-capped test tubes (larger diameter)
* use a blank containing your media
* use a blank containing your media
* ok to leave on all day, but do turn it off when done  
* ok to leave on all day, but do turn it off when done  
** bulbs are cheap  (UV bulbs are generally the expensive ones)
** bulbs are cheap  (UV bulbs are generally the expensive ones)
 +
 +
==To Use==
 +
# Turn on by turning leftmost dial clockwise
 +
# Set filter to 600 nm (or whatever)
 +
# Zero transmittance with left knob
 +
# Put in blank & cover with black cap
 +
# set transmittance to 100
 +
# absorbance for the blank should now be 0
 +
# Measure absorbance for your sample
 +
 +
* Keep tube orientation the same each time you measure in case of any unevenness. 
[http://www.cienytec.com/PDFS/Espec_SPEC20_OpMan_ing.pdf manual]
[http://www.cienytec.com/PDFS/Espec_SPEC20_OpMan_ing.pdf manual]

Revision as of 19:40, 7 June 2012

Return: Protocols

Tube Spec

  • minimum volume: 5 mL
  • use green-capped test tubes (larger diameter)
  • use a blank containing your media
  • ok to leave on all day, but do turn it off when done
    • bulbs are cheap (UV bulbs are generally the expensive ones)

To Use

  1. Turn on by turning leftmost dial clockwise
  2. Set filter to 600 nm (or whatever)
  3. Zero transmittance with left knob
  4. Put in blank & cover with black cap
  5. set transmittance to 100
  6. absorbance for the blank should now be 0
  7. Measure absorbance for your sample
  • Keep tube orientation the same each time you measure in case of any unevenness.


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