Lidstrom:Thermocyclers: Difference between revisions
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== Machines available as of 6/2013 == | |||
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Revision as of 08:40, 13 June 2013
Back to Protocols
Machines available as of 6/2013
| Thermocycler location | Appearance | Brand | Capabilities | Manual | Use restrictions |
| Janet's bench | black | DNA Engine: DYAD | small block doesn't do gradient; large block does | n/a | none |
| Amanda's window | white & new | Eppendorf: Mastercycler gradient | gradient, but see manual b/c it is weird | Mastercycler Gradient | none |
| Frances' window | black & old | DNA engine | no gradient | n/a | none |
| Nate's Bench | ## | ## | ## | ## | none? |
| Alexey's Bench | ## | ## | 2 blocks (gradient?) | ## | none? |
| Ceci's window | white, small | Eppendorf: ?? | ?? | ?? | none? |
What makes for a good thermocycler?
Functions
- gradient block(s)
- multiple blocks on one machine
- reduces the need to enter programs into many machines
- allow for saving of protocols in folders
- different researchers can have different folders, preventing the accumulation of a huge list of protocols, and others accidentally writing over other peoples' protocols
Interface
- intuitive menu
- NOT true of eppendorf systems!
- BioRad system is fantastic
- Show what happens to each well for a given gradient setting *while* the protocol is being edited
- Not true of white eppendorf Mastercycler gradient
Bonus Points
- ability to recall past thermocycling programs used
- occasionally useful for troubleshooting failed reactions
