Lidstrom:TB - Revision history

Lidstrom:TB - Revision history http://openwetware.org/index.php?title=Lidstrom:TB&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Wed, 22 May 2013 17:42:53 GMT Amanda L Smith at 23:11, 17 April 2013 http://openwetware.org/index.php?title=Lidstrom:TB&diff=692455&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:11, 17 April 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**"Glycerol play the part of a cheap carbon-energy source for E.coli.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**"Glycerol play the part of a cheap carbon-energy source for E.coli.  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*** Ecoli grown on glycerol excretes less acetate compared to e coli grown on glucose. (due to changes in the biochemical pathways and slower absorption rate of glycerol). Acetate is bad because it limits the growth of e coli. This is due primarily by methionine synthesis inhibition, which in turn disrupts protein synthesis and alters protein expression (A horror story if you are expressing proteins). Acetate also acidifies the media which also cause other changes in E coli physiology (such as reduced growth rate, altered K/Na ion and amino acid anions concentration. Which also has effects on protein synthesis)"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*** Ecoli grown on glycerol excretes less acetate compared to e coli grown on glucose. (due to changes in the biochemical pathways and slower absorption rate of glycerol). Acetate is bad because it limits the growth of e coli. This is due primarily by methionine synthesis inhibition, which in turn disrupts protein synthesis and alters protein expression (A horror story if you are expressing proteins). Acetate also acidifies the media which also cause other changes in E coli physiology (such as reduced growth rate, altered K/Na ion and amino acid anions concentration. Which also has effects on protein synthesis)"</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">***So, I just read something that disagreed with the common knowledge that acetate limits E. coli growth and interferes with protein expression. Will post if I can find.</ins></div></td></tr>  </table> Wed, 17 Apr 2013 23:11:02 GMT Amanda L Smith http://openwetware.org/wiki/Talk:Lidstrom:TB Janet B. Matsen at 18:23, 21 February 2013 http://openwetware.org/index.php?title=Lidstrom:TB&diff=678298&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:23, 21 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Return: [[Lidstrom:Protocols|Protocols]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Return: [[Lidstrom:Protocols|Protocols]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Terrific Broth]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Recipe: ==</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* We use the OWW recipe: </ins>[[Terrific Broth]]</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">== Ever wonder why we feed them glycerol, not glucose? ==</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* [[User:Amanda_L_Smith|Amanda Smith]] found: </ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">**"Glycerol play the part of a cheap carbon-energy source for E.coli. </ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*** Ecoli grown on glycerol excretes less acetate compared to e coli grown on glucose. (due to changes in the biochemical pathways and slower absorption rate of glycerol). Acetate is bad because it limits the growth of e coli. This is due primarily by methionine synthesis inhibition, which in turn disrupts protein synthesis and alters protein expression (A horror story if you are expressing proteins). Acetate also acidifies the media which also cause other changes in E coli physiology (such as reduced growth rate, altered K/Na ion and amino acid anions concentration. Which also has effects on protein synthesis)"</ins></div></td></tr>  </table> Thu, 21 Feb 2013 18:23:34 GMT Janet B. Matsen http://openwetware.org/wiki/Talk:Lidstrom:TB Amanda L Smith: New page: Return: Protocols Terrific Broth http://openwetware.org/index.php?title=Lidstrom:TB&diff=612148&oldid=prev <p>New page: Return: <a href="/wiki/Lidstrom:Protocols" title="Lidstrom:Protocols">Protocols</a> <a href="/wiki/Terrific_Broth" title="Terrific Broth">Terrific Broth</a></p> <p><b>New page</b></p><div>Return: [[Lidstrom:Protocols|Protocols]]<br /> <br /> [[Terrific Broth]]</div> Tue, 03 Jul 2012 21:25:42 GMT Amanda L Smith http://openwetware.org/wiki/Talk:Lidstrom:TB