Lidstrom:Sorvall Centrifuge

From OpenWetWare
Revision as of 10:19, 24 October 2013 by Janet B. Matsen (talk | contribs)
Jump to navigationJump to search

Back to Protocols


Picture

Lidstrom Sorvoll centrifuge

Rotors

  • Sorvall rotors
    • Rotors we have:
      • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
      • GS-3.
      • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation

Centrifugation Math Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Lidstrom:Sorvall_Centrifuge&oldid=743999"