Lidstrom:Sorvall Centrifuge

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(Rotors)
(Rotors)
Line 9: Line 9:
** Rotors we have:
** Rotors we have:
*** SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
*** SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
-
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.5|center|SS-34 tubes]]. Can't be autoclaved.  Can't write on body; can only write on cap.  Sandy says syickers don't stick.  
+
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] Can't be autoclaved.  Can't write on body; can only write on cap.  Sandy says syickers don't stick.  
*** GS-3.
*** GS-3.
*** ____.  Holds eppendorf (1.5mL) tubes
*** ____.  Holds eppendorf (1.5mL) tubes

Revision as of 12:39, 24 October 2013

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Contents

Picture

Lidstrom Sorvoll centrifuge
Lidstrom Sorvoll centrifuge

Rotors

  • Sorvall rotors
    • Rotors we have:
      • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
        • Tubes:
          SS-34 tubes
          SS-34 tubes
          Can't be autoclaved. Can't write on body; can only write on cap. Sandy says syickers don't stick.
      • GS-3.
      • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Lidstrom sorvoll speed instructions
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
      Stoke's equation
    • centrifugation speeds for applications
      centrifugation speeds for applications
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