Lidstrom:Sorvall Centrifuge: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Rotors) |
|||
| Line 9: | Line 9: | ||
** Rotors we have: | ** Rotors we have: | ||
*** SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | *** SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | ||
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=2.0|center|SS-34 tubes]] | |||
*** GS-3. | *** GS-3. | ||
*** ____. Holds eppendorf (1.5mL) tubes | *** ____. Holds eppendorf (1.5mL) tubes | ||
Revision as of 10:37, 24 October 2013
Back to Protocols
Picture
Rotors
- Sorvall rotors
- Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Tubes:
SS-34 tubes
- Tubes:
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Rotors we have:
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
Stoke's equation 
centrifugation speeds for applications
