Lidstrom:Sorvall Centrifuge

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Centrifugation Math Basics)
(Centrifugation Math & Basics)
Line 20: Line 20:
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
 +
 +
== Instructions ==
 +
Example protocol for AM1:
 +
*
 +
 +
Janet's SIP3-4 centrifugation:
 +
* Chill to 4<sup>o</sup>C with SS-34 rotor inside
 +
* Fill correct tubes
 +
* Spin for ___ min at ___ RCF at ___<sup>o</sup>C
== Centrifugation Math & Basics ==
== Centrifugation Math & Basics ==

Revision as of 13:28, 24 October 2013

Back to Protocols


Contents

Picture

Lidstrom Sorvoll centrifuge
Lidstrom Sorvoll centrifuge

Rotors

  • Sorvall rotors
    • Rotors we have:
      • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
      • GS-3.
      • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Lidstrom sorvoll speed instructions
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
      Stoke's equation
    • centrifugation speeds for applications
      centrifugation speeds for applications
Personal tools