Lidstrom:Sorvall Centrifuge: Difference between revisions
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[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | ||
== Centrifugation Math Basics == | == Centrifugation Math & Basics == | ||
* [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]: | * [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]: | ||
** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter). | ** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter). | ||
** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]] | ** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]] | ||
** [[image:Lidstrom centrifugation speeds for applications.png|thumb|upright=2.0|center|centrifugation speeds for applications]] | |||
Revision as of 10:21, 24 October 2013
Back to Protocols
Picture
Rotors
- Sorvall rotors
- Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes
- Rotors we have:
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
Stoke's equation 
centrifugation speeds for applications
