Lidstrom:Sorvall Centrifuge: Difference between revisions
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**** Actually, Amanda might have autoclaved them before. | **** Actually, Amanda might have autoclaved them before. | ||
***Can't write on body; can only write on cap. Sandy says stickers don't stick. | ***Can't write on body; can only write on cap. Sandy says stickers don't stick. | ||
=== GS-3 === | |||
* [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3]. | * [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3]. | ||
** For bigger bottles. | ** For bigger bottles. (0.5L) | ||
** Holds eppendorf (1.5mL) tubes | ** Holds eppendorf (1.5mL) tubes | ||
[[image:SS-34 rotor speeds and k factors.png|thumb|upright=2.0|center|SS-34 rotor speeds and k factors]] | [[image:SS-34 rotor speeds and k factors.png|thumb|upright=2.0|center|SS-34 rotor speeds and k factors]] | ||
[[image:GS-3 rotor speeds and k factors.png|thumb|upright=2.0|center|GS-34 rotor speeds and k factors]] | |||
== Explanation of RCF == | |||
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | |||
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF. | |||
*There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed. | |||
[[image: | [[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]] | ||
== Instructions == | == Instructions == |
Revision as of 17:47, 21 January 2015
Back to Protocols
Picture
Rotors
SS-34
- Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Tubes:
- Can't be autoclaved.
- Actually, Amanda might have autoclaved them before.
- Can't write on body; can only write on cap. Sandy says stickers don't stick.
- Can't be autoclaved.
- Tubes:
GS-3
- GS-3.
- For bigger bottles. (0.5L)
- Holds eppendorf (1.5mL) tubes
Explanation of RCF
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
- There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).