Lidstrom:Sorvall Centrifuge: Difference between revisions

Revision as of 17:45, 21 January 2015

Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Tubes:
  SS-34 tubes
  - Can't be autoclaved.
    - Actually, Amanda might have autoclaved them before.
  - Can't write on body; can only write on cap. Sandy says stickers don't stick.
GS-3.
- For bigger bottles.
???.
- Holds eppendorf (1.5mL) tubes
For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.

There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
- Stoke's equation
- centrifugation speeds for applications

@@ Line 8: / Line 8: @@
 [http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
-Rotors we have:
-* SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
+=== SS-34 ===
+* Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
 **Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]]
 ***Can't be autoclaved.
@@ Line 25: / Line 26: @@
 [[image:SS-34 rotor speeds and k factors.png|thumb|upright=2.0|center|SS-34 rotor speeds and k factors]]
+=== GS-3 ===
 [[image:GS-3 rotor speeds and k factors.png|thumb|upright=2.0|center|GS-34 rotor speeds and k factors]]
-[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
 == Instructions ==