Lidstrom:Sorvall Centrifuge

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Rotors)
Current revision (20:03, 11 June 2014) (view source)
(Rotors)
 
(11 intermediate revisions not shown.)
Line 6: Line 6:
== Rotors ==
== Rotors ==
-
* [http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
+
[http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
-
** Rotors we have:
+
 
-
*** SS-34.  Holds 8*50 mL, spins at ___ RPM/____ RCF
+
Rotors we have:
-
*** GS-3.
+
* SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
-
*** ____.  Holds eppendorf (1.5mL) tubes
+
**Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]]
 +
***Can't be autoclaved. 
 +
**** Actually, Amanda might have autoclaved them before. 
 +
***Can't write on body; can only write on cap.  Sandy says stickers don't stick.
 +
* [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3].
 +
** For bigger bottles.
 +
* ???.   
 +
** Holds eppendorf (1.5mL) tubes
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
 +
*There are stickers with maximum speeds.  These were set by Katya, a Lidstrom Lab manager, circa 2011.  It is unknown what the motivation was for keeping the rotors below maximum speed.
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
Line 20: Line 28:
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
 +
 +
== Instructions ==
 +
Example protocol for AM1:
 +
*
 +
 +
Janet's SIP3-4 centrifugation:
 +
* Chill to 4<sup>o</sup>C with SS-34 rotor inside
 +
* Fill correct tubes
 +
* Spin for ___ min at ___ RCF at ___<sup>o</sup>C
 +
 +
== Centrifugation Math & Basics ==
 +
* [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]:
 +
** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
 +
** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]]
 +
** [[image:Lidstrom centrifugation speeds for applications.png|thumb|upright=2.0|center|centrifugation speeds for applications]]

Current revision

Back to Protocols


Contents

Picture

Lidstrom Sorvoll centrifuge
Lidstrom Sorvoll centrifuge

Rotors

Sorvall rotors

Rotors we have:

  • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
    • Tubes:
      SS-34 tubes
      SS-34 tubes
      • Can't be autoclaved.
        • Actually, Amanda might have autoclaved them before.
      • Can't write on body; can only write on cap. Sandy says stickers don't stick.
  • GS-3.
    • For bigger bottles.
  •  ???.
    • Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
  • There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Lidstrom sorvoll speed instructions
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
      Stoke's equation
    • centrifugation speeds for applications
      centrifugation speeds for applications
Personal tools