Lidstrom:Sorvall Centrifuge: Difference between revisions
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== Rotors == | == Rotors == | ||
[http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors] | |||
Rotors we have: | |||
* SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | |||
**Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] | |||
***Can't be autoclaved. | |||
**** Actually, Amanda might have autoclaved them before. | |||
***Can't write on body; can only write on cap. Sandy says stickers don't stick. | |||
* GS-3. | |||
* ____. Holds eppendorf (1.5mL) tubes | |||
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF. | * For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF. | ||
There are stickers with maximum speeds. These were set by Katya, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed. | *There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed. | ||
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]] | [[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]] | ||
Revision as of 17:00, 11 June 2014
Back to Protocols
Picture
Rotors
Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Tubes:
SS-34 tubes - Can't be autoclaved.
- Actually, Amanda might have autoclaved them before.
- Can't write on body; can only write on cap. Sandy says stickers don't stick.
- Can't be autoclaved.
- Tubes:
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
- There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
Stoke's equation 
centrifugation speeds for applications
