Lidstrom:Sorvall Centrifuge

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** Rotors we have:
** Rotors we have:
*** SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
*** SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
 +
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] Can't be autoclaved.  Can't write on body; can only write on cap.  Sandy says syickers don't stick.
*** GS-3.
*** GS-3.
*** ____.  Holds eppendorf (1.5mL) tubes
*** ____.  Holds eppendorf (1.5mL) tubes
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
 +
There are stickers with maximum speeds.  These were set by Katya, circa 2011.  It is unknown what the motivation was for keeping the rotors below maximum speed.
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
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[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
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== Instructions ==
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Example protocol for AM1:
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*
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Janet's SIP3-4 centrifugation:
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* Chill to 4<sup>o</sup>C with SS-34 rotor inside
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* Fill correct tubes
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* Spin for ___ min at ___ RCF at ___<sup>o</sup>C
== Centrifugation Math & Basics ==
== Centrifugation Math & Basics ==

Revision as of 12:44, 24 October 2013

Back to Protocols


Contents

Picture

Lidstrom Sorvoll centrifuge
Lidstrom Sorvoll centrifuge

Rotors

  • Sorvall rotors
    • Rotors we have:
      • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
        • Tubes:
          SS-34 tubes
          SS-34 tubes
          Can't be autoclaved. Can't write on body; can only write on cap. Sandy says syickers don't stick.
      • GS-3.
      • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.

There are stickers with maximum speeds. These were set by Katya, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.

Lidstrom sorvoll speed instructions
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
      Stoke's equation
    • centrifugation speeds for applications
      centrifugation speeds for applications
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