Lidstrom:Sorvall Centrifuge: Difference between revisions

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Back to [[Lidstrom:Protocols|Protocols]]
Back to [[Lidstrom:Protocols|Protocols]]


== Manuals ==
 
== Picture ==
[[image:Lidstrom_Sorvoll.png|thumb|upright=1.0|center|Lidstrom Sorvoll centrifuge]]
 
== Rotors ==
* [http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
* [http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
** Rotors we have:
** Rotors we have:
*** SS-34.  Holds 8*50 mL, spins at ___ RPM/____ RCF
*** SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] Can't be autoclaved.  Can't write on body; can only write on cap.  Sandy says syickers don't stick.
*** GS-3.
*** GS-3.
*** ____.  Holds eppendorf (1.5mL) tubes
*** ____.  Holds eppendorf (1.5mL) tubes
[[image:Lidstrom_Sorvoll.png|thumb|upright=1.0|center|Lidstrom Sorvoll centrifuge]]
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.
 
There are stickers with maximum speeds.  These were set by Katya, circa 2011.  It is unknown what the motivation was for keeping the rotors below maximum speed.
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
 
[[image:SS-34 rotor speeds and k factors.png|thumb|upright=2.0|center|SS-34 rotor speeds and k factors]]
 
[[image:GS-3 rotor speeds and k factors.png|thumb|upright=2.0|center|GS-34 rotor speeds and k factors]]
 
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]


[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=3.0|center|Lidstrom sorvoll speed instructions]]
== Instructions ==
Example protocol for AM1:
*


[[image:SS-34 rotor speeds and k factors.png|thumb|upright=3.0|center|SS-34 rotor speeds and k factors]]
Janet's SIP3-4 centrifugation:
* Chill to 4<sup>o</sup>C with SS-34 rotor inside
* Fill correct tubes
* Spin for ___ min at ___ RCF at ___<sup>o</sup>C


[[image:GS-34 rotor speeds and k factors.png|thumb|upright=3.0|center|GS-34 rotor speeds and k factors]]
== Centrifugation Math & Basics ==
* [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]:
** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]]
** [[image:Lidstrom centrifugation speeds for applications.png|thumb|upright=2.0|center|centrifugation speeds for applications]]

Revision as of 10:44, 24 October 2013

Back to Protocols


Picture

Lidstrom Sorvoll centrifuge

Rotors

  • Sorvall rotors
    • Rotors we have:
      • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
        • Tubes:
          SS-34 tubes
          Can't be autoclaved. Can't write on body; can only write on cap. Sandy says syickers don't stick.
      • GS-3.
      • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.

There are stickers with maximum speeds. These were set by Katya, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.

Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
    • centrifugation speeds for applications
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