Lidstrom:Sorvall Centrifuge: Difference between revisions
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** Rotors we have: | ** Rotors we have: | ||
*** SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | *** SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | ||
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0. | ****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] Can't be autoclaved. Can't write on body; can only write on cap. Sandy says syickers don't stick. | ||
*** GS-3. | *** GS-3. | ||
*** ____. Holds eppendorf (1.5mL) tubes | *** ____. Holds eppendorf (1.5mL) tubes |
Revision as of 10:39, 24 October 2013
Back to Protocols
Picture
Rotors
- Sorvall rotors
- Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Tubes: Can't be autoclaved. Can't write on body; can only write on cap. Sandy says syickers don't stick.
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Rotors we have:
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).