# Lidstrom:Sorvall Centrifuge

(Difference between revisions)
 Revision as of 13:21, 24 October 2013 (view source) (→Centrifugation Math Basics)← Previous diff Revision as of 13:28, 24 October 2013 (view source) (→Centrifugation Math & Basics)Next diff → Line 20: Line 20: [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] + + == Instructions == + Example protocol for AM1: + * + + Janet's SIP3-4 centrifugation: + * Chill to 4oC with SS-34 rotor inside + * Fill correct tubes + * Spin for ___ min at ___ RCF at ___oC == Centrifugation Math & Basics == == Centrifugation Math & Basics ==

## Revision as of 13:28, 24 October 2013

Back to Protocols

## Picture

Lidstrom Sorvoll centrifuge

## Rotors

• Sorvall rotors
• Rotors we have:
• SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
• GS-3.
• ____. Holds eppendorf (1.5mL) tubes
• For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation

## Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

• Chill to 4oC with SS-34 rotor inside
• Fill correct tubes
• Spin for ___ min at ___ RCF at ___oC

## Centrifugation Math & Basics

• Sigma summary:
• sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
• Stoke's equation
• centrifugation speeds for applications