# Lidstrom:Sorvall Centrifuge

(Difference between revisions)
 Revision as of 13:19, 24 October 2013 (view source)← Previous diff Revision as of 13:21, 24 October 2013 (view source) (→Centrifugation Math Basics)Next diff → Line 21: Line 21: [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] - == Centrifugation Math Basics == + == Centrifugation Math & Basics == * [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]: * [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]: ** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter). ** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter). ** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]] ** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]] + ** [[image:Lidstrom centrifugation speeds for applications.png|thumb|upright=2.0|center|centrifugation speeds for applications]]

## Revision as of 13:21, 24 October 2013

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## Picture

Lidstrom Sorvoll centrifuge

## Rotors

• Sorvall rotors
• Rotors we have:
• SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
• GS-3.
• ____. Holds eppendorf (1.5mL) tubes
• For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation

## Centrifugation Math & Basics

• Sigma summary:
• sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
• Stoke's equation
• centrifugation speeds for applications