Lidstrom:Sorvall Centrifuge

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(Rotors)
(Rotors)
Line 14: Line 14:
**** Actually, Amanda might have autoclaved them before.   
**** Actually, Amanda might have autoclaved them before.   
***Can't write on body; can only write on cap.  Sandy says stickers don't stick.  
***Can't write on body; can only write on cap.  Sandy says stickers don't stick.  
-
* GS-3.
+
* [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3].
 +
** For bigger bottles.  
* ____.  Holds eppendorf (1.5mL) tubes
* ____.  Holds eppendorf (1.5mL) tubes
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  

Revision as of 19:02, 11 June 2014

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Contents

Picture

Lidstrom Sorvoll centrifuge
Lidstrom Sorvoll centrifuge

Rotors

Sorvall rotors

Rotors we have:

  • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
    • Tubes:
      SS-34 tubes
      SS-34 tubes
      • Can't be autoclaved.
        • Actually, Amanda might have autoclaved them before.
      • Can't write on body; can only write on cap. Sandy says stickers don't stick.
  • GS-3.
    • For bigger bottles.
  • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
  • There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Lidstrom sorvoll speed instructions
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
      Stoke's equation
    • centrifugation speeds for applications
      centrifugation speeds for applications
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