Lidstrom:Reducing Agents: Difference between revisions
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(New page: == Why are reducing agents used? == == What reducing agents are commonly used? == * DTT * b-ME (β-mercaptoethanol) * TCEP ** more expensive, odorless == Misc == * [http://info.agscienti...) |
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* [http://info.agscientific.com/blog/bid/101786/Side-by-Side-comparison-DTT-VS-TCEP-Preferred-Reducing-Reagents Side by Side comparison, DTT VS TCEP] | * [http://info.agscientific.com/blog/bid/101786/Side-by-Side-comparison-DTT-VS-TCEP-Preferred-Reducing-Reagents Side by Side comparison, DTT VS TCEP] | ||
** TCEP is not very stable in phosphate buffers, especially at neutral pH. | ** TCEP is not very stable in phosphate buffers, especially at neutral pH. | ||
** Effective pH range is 1.5 to 8.5. | |||
Revision as of 13:35, 24 April 2014
Why are reducing agents used?
What reducing agents are commonly used?
- DTT
- b-ME (β-mercaptoethanol)
- TCEP
- more expensive, odorless
Misc
- Side by Side comparison, DTT VS TCEP
- TCEP is not very stable in phosphate buffers, especially at neutral pH.
- Effective pH range is 1.5 to 8.5.
