Lidstrom:Protocols - Revision history

Janet B. Matsen: /* Cell Culture: */

Janet B. Matsen — Thu, 02 May 2013 22:24:05 GMT

Cell Culture:

←Older revision		Revision as of 22:24, 2 May 2013
Line 13:		Line 13:
	==Cell Culture:==		==Cell Culture:==
	*[[Lidstrom:Tube Spec\|Tube Spec]]		*[[Lidstrom:Tube Spec\|Tube Spec]]
		+	*[[Lidstrom: Bioscreen\|Bioscreen Setup]]
		+	=== Methylotrophs ===
		+	*[[Lidstrom:MM1/MM2/MM3/HY\|MM1/MM2/MM3/HY]]
		+	===E. coli ===
	*[[Lidstrom:M9\|M9 recipe]]		*[[Lidstrom:M9\|M9 recipe]]
	*[[Lidstrom:TB\|Terrific Broth recipe]]		*[[Lidstrom:TB\|Terrific Broth recipe]]
-	*[[Lidstrom: Bioscreen\|Bioscreen Setup]]
	*[[Lidstrom: EZ amino acid stock recipe\|EZ amino acid stock recipe]]		*[[Lidstrom: EZ amino acid stock recipe\|EZ amino acid stock recipe]]

Janet B. Matsen: /* DNA */

Janet B. Matsen — Sun, 31 Mar 2013 14:07:40 GMT

DNA

←Older revision		Revision as of 14:07, 31 March 2013
Line 30:		Line 30:
	*[[Lidstrom:Overlap Extension PCR\| Overlap Extension PCR (in progress)]]		*[[Lidstrom:Overlap Extension PCR\| Overlap Extension PCR (in progress)]]
	*[[Qiagen_Buffers\| Qiagen Buffer Recipes]]		*[[Qiagen_Buffers\| Qiagen Buffer Recipes]]
		+
		+	=====Fancy Manipulation=====
		+	*[[Lidstrom:Site-Directed Mutagenesis\|Site-Directed Mutagenesis (in progress)]]
		+	*[[Lidstrom:λ-Red Mediated Gene Knockouts\|λ-Red Mediated Gene Knockouts (in progress)]]
		+	*[[Dorman:P1_phage_%28lysogenic%29\|Phage Lysate Prep]]
		+	*[[Lidstrom:P1 Phage Transduction\|P1 Phage Transduction (in progress)]]

	==== Restriction Cloning ====		==== Restriction Cloning ====
Line 37:		Line 43:
	==== Gibson Cloning ====		==== Gibson Cloning ====
	*[[Lidstrom:Gibson Assembly\| Gibson Assembly (In progress)]]		*[[Lidstrom:Gibson Assembly\| Gibson Assembly (In progress)]]
-
-	~~=====Fancy Manipulation=====~~
-	*[[Lidstrom:Site-Directed Mutagenesis\|Site-Directed Mutagenesis (in progress)]]
-	*[[Lidstrom:λ-Red Mediated Gene Knockouts\|λ-Red Mediated Gene Knockouts (in progress)]]
-	*[[Dorman:P1_phage_%28lysogenic%29\|Phage Lysate Prep]]
-	*[[Lidstrom:P1 Phage Transduction\|P1 Phage Transduction (in progress)]]

	====Obervation====		====Obervation====

Janet B. Matsen: /* DNA/RNA: */

Janet B. Matsen — Sun, 31 Mar 2013 14:07:02 GMT

DNA/RNA:

←Older revision		Revision as of 14:07, 31 March 2013
Line 28:		Line 28:
	====Manipulation====		====Manipulation====
	*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]		*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]
-	*[[Lidstrom:Digestion with Restriction Enzymes\|Digestion with Restriction Enzymes]]
-	*[[Lidstrom:Gibson Assembly\| Gibson Assembly (In progress)]]
-	*[[Lidstrom:Ligation \|Ligation (in progress)]]
	*[[Lidstrom:Overlap Extension PCR\| Overlap Extension PCR (in progress)]]		*[[Lidstrom:Overlap Extension PCR\| Overlap Extension PCR (in progress)]]
	*[[Qiagen_Buffers\| Qiagen Buffer Recipes]]		*[[Qiagen_Buffers\| Qiagen Buffer Recipes]]
		+
		+	==== Restriction Cloning ====
		+	*[[Lidstrom:Digestion with Restriction Enzymes\|Digestion with Restriction Enzymes]]
		+	*[[Lidstrom:Ligation \|Ligation (in progress)]]
		+
		+	==== Gibson Cloning ====
		+	*[[Lidstrom:Gibson Assembly\| Gibson Assembly (In progress)]]

	=====Fancy Manipulation=====		=====Fancy Manipulation=====

Nicole Smalley: /* Isolation */

Nicole Smalley — Tue, 26 Feb 2013 18:43:27 GMT

Isolation

←Older revision		Revision as of 18:43, 26 February 2013
Line 23:		Line 23:
	====Isolation ====		====Isolation ====
	*[[Lidstrom:QIAquick Gel Extraction Kit Protocol\|QIAquick Gel Extraction Kit Protocol]]		*[[Lidstrom:QIAquick Gel Extraction Kit Protocol\|QIAquick Gel Extraction Kit Protocol]]
-	*[[Lidstrom:Genomic DNA extraction\|Genomic DNA extraction]]	+	*[[Lidstrom:Genomic DNA extraction\|Genomic DNA minikit extraction]]
		+	*[[Lidstrom:Genomic DNA extraction\|Genomic DNA phenol/chloroform extraction]]

	====Manipulation====		====Manipulation====

Janet B. Matsen: /* Cell Culture: */

Janet B. Matsen — Mon, 11 Feb 2013 02:25:46 GMT

Cell Culture:

←Older revision		Revision as of 02:25, 11 February 2013
Line 16:		Line 16:
	*[[Lidstrom:TB\|Terrific Broth recipe]]		*[[Lidstrom:TB\|Terrific Broth recipe]]
	*[[Lidstrom: Bioscreen\|Bioscreen Setup]]		*[[Lidstrom: Bioscreen\|Bioscreen Setup]]
		+	*[[Lidstrom: EZ amino acid stock recipe\|EZ amino acid stock recipe]]

	==DNA/RNA:==		==DNA/RNA:==

Janet B. Matsen: /* Isolation */

Janet B. Matsen — Mon, 04 Feb 2013 23:45:18 GMT

Isolation

←Older revision		Revision as of 23:45, 4 February 2013
Line 22:		Line 22:
	====Isolation ====		====Isolation ====
	*[[Lidstrom:QIAquick Gel Extraction Kit Protocol\|QIAquick Gel Extraction Kit Protocol]]		*[[Lidstrom:QIAquick Gel Extraction Kit Protocol\|QIAquick Gel Extraction Kit Protocol]]
		+	*[[Lidstrom:Genomic DNA extraction\|Genomic DNA extraction]]
		+
	====Manipulation====		====Manipulation====
	*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]		*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]

Janet B. Matsen: /* DNA */

Janet B. Matsen — Mon, 04 Feb 2013 23:30:56 GMT

DNA

←Older revision		Revision as of 23:30, 4 February 2013
Line 20:		Line 20:

	===DNA===		===DNA===
-		+	====Isolation ====
		+	*[[Lidstrom:QIAquick Gel Extraction Kit Protocol\|QIAquick Gel Extraction Kit Protocol]]
	====Manipulation====		====Manipulation====
	*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]		*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]

Amanda L Smith at 21:53, 1 February 2013

Amanda L Smith — Fri, 01 Feb 2013 21:53:54 GMT

←Older revision		Revision as of 21:53, 1 February 2013
Line 10:		Line 10:
	*[[Lidstrom:Preparing Freezer Cell Stocks\| Preparing Freezer Cell Stocks]]		*[[Lidstrom:Preparing Freezer Cell Stocks\| Preparing Freezer Cell Stocks]]
	*[[Lidstrom:Pouring Media Plates\|Pouring Media Plates]]		*[[Lidstrom:Pouring Media Plates\|Pouring Media Plates]]
-	*[[Lidstrom:Ordering \| Ordering]]

	==Cell Culture:==		==Cell Culture:==

Amanda L Smith: /* General Lab: */

Amanda L Smith — Fri, 01 Feb 2013 21:48:33 GMT

General Lab:

←Older revision		Revision as of 21:48, 1 February 2013
Line 10:		Line 10:
	*[[Lidstrom:Preparing Freezer Cell Stocks\| Preparing Freezer Cell Stocks]]		*[[Lidstrom:Preparing Freezer Cell Stocks\| Preparing Freezer Cell Stocks]]
	*[[Lidstrom:Pouring Media Plates\|Pouring Media Plates]]		*[[Lidstrom:Pouring Media Plates\|Pouring Media Plates]]
		+	*[[Lidstrom:Ordering \| Ordering]]

	==Cell Culture:==		==Cell Culture:==

Janet B. Matsen: /* Metabolomics */

Janet B. Matsen — Tue, 20 Nov 2012 20:21:32 GMT

Metabolomics

←Older revision		Revision as of 20:21, 20 November 2012
Line 69:		Line 69:

	== Metabolomics ==		== Metabolomics ==
		+	=== CO2 project ===
	*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project\|Measuring 13C Incorporation Into Protein - CO2 Project]]		*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project\|Measuring 13C Incorporation Into Protein - CO2 Project]]
		+	** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

←Older revision		Revision as of 22:24, 2 May 2013
Line 13:		Line 13:
	==Cell Culture:==		==Cell Culture:==
	*[[Lidstrom:Tube Spec\|Tube Spec]]		*[[Lidstrom:Tube Spec\|Tube Spec]]
		+	*[[Lidstrom: Bioscreen\|Bioscreen Setup]]
		+	=== Methylotrophs ===
		+	*[[Lidstrom:MM1/MM2/MM3/HY\|MM1/MM2/MM3/HY]]
		+	===E. coli ===
	*[[Lidstrom:M9\|M9 recipe]]		*[[Lidstrom:M9\|M9 recipe]]
	*[[Lidstrom:TB\|Terrific Broth recipe]]		*[[Lidstrom:TB\|Terrific Broth recipe]]
-	*[[Lidstrom: Bioscreen\|Bioscreen Setup]]
	*[[Lidstrom: EZ amino acid stock recipe\|EZ amino acid stock recipe]]		*[[Lidstrom: EZ amino acid stock recipe\|EZ amino acid stock recipe]]

←Older revision		Revision as of 14:07, 31 March 2013
Line 28:		Line 28:
	====Manipulation====		====Manipulation====
	*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]		*[[Lidstrom:Building with DNA\|Intro: Building with DNA (in progress)]]
-	*[[Lidstrom:Digestion with Restriction Enzymes\|Digestion with Restriction Enzymes]]
-	*[[Lidstrom:Gibson Assembly\| Gibson Assembly (In progress)]]
-	*[[Lidstrom:Ligation \|Ligation (in progress)]]
	*[[Lidstrom:Overlap Extension PCR\| Overlap Extension PCR (in progress)]]		*[[Lidstrom:Overlap Extension PCR\| Overlap Extension PCR (in progress)]]
	*[[Qiagen_Buffers\| Qiagen Buffer Recipes]]		*[[Qiagen_Buffers\| Qiagen Buffer Recipes]]
		+
		+	==== Restriction Cloning ====
		+	*[[Lidstrom:Digestion with Restriction Enzymes\|Digestion with Restriction Enzymes]]
		+	*[[Lidstrom:Ligation \|Ligation (in progress)]]
		+
		+	==== Gibson Cloning ====
		+	*[[Lidstrom:Gibson Assembly\| Gibson Assembly (In progress)]]

	=====Fancy Manipulation=====		=====Fancy Manipulation=====