Lidstrom:Protocols

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(Cell Culture:)
Current revision (10:50, 4 September 2014) (view source)
(Fancy Manipulation)
 
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=====Fancy Manipulation=====
=====Fancy Manipulation=====
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
-
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
+
** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]]
 +
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
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*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
 +
 +
====Mutagenesis====
 +
*[[Lidstrom:EMS Mutagenesis|EMS Mutagenesis]]
 +
*[[Lidstrom:UV Mutagenesis|UV Mutagenesis]]
====Misc.====
====Misc.====
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==Protein==
==Protein==
 +
=== Purification ===
 +
*[[Lidstrom:His-tag Protein Purification|His-tag Protein Purification]]
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 +
=== Analytics ===
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
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*[[Lidstrom:French Press|French Press]]
*[[Lidstrom:French Press|French Press]]
*[[Lidstrom:Sonicator|Sonication]]
*[[Lidstrom:Sonicator|Sonication]]
 +
*[[Lidstrom:Bug Buster|Bug Buster]]
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
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===General===
===General===
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
 +
*[[Lidstrom:Enzyme Assay Data Analysis|Enzyme Assay Data Analysis]]
*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
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*[[Lidstrom:Path Length in microwell plates|Path Length in 96-well plates]]
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*[[Lidstrom:Reducing Agents|Reducing Agents]]
=== Equipment ===
=== Equipment ===
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==== Misc ====
==== Misc ====
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
 +
*[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]]
== Metabolomics ==
== Metabolomics ==

Current revision

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Mutagenesis

Misc.

Protein

Purification

Analytics

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project

Personal tools