Lidstrom:Protocols: Difference between revisions
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*[[Lidstrom:Autoclave |Autoclave]] | *[[Lidstrom:Autoclave |Autoclave]] | ||
*[[Lidstrom:Sterile Technique | Sterile technique]] | *[[Lidstrom:Sterile Technique | Sterile technique]] | ||
*[[Lidstrom:E. | *[[Lidstrom:E. coli basics|E. coli basics]] | ||
*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]] | *[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]] | ||
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]] | *[[Lidstrom:Pouring Media Plates|Pouring Media Plates]] | ||
| Line 48: | Line 48: | ||
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | *[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | ||
** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]] | ** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]] | ||
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts | *[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts]] | ||
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | *[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | ||
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | *[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | ||
| Line 96: | Line 96: | ||
==E. coli== | ==E. coli== | ||
*[[Lidstrom:E. | *[[Lidstrom:E. coli Vector Compatibility| E. coli Vector Compatibility (In Progress)]] | ||
** origins of replication | |||
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | *[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | ||
=== E. coli transformation === | === E. coli transformation === | ||
*[[Lidstrom:Chemical Transformation|Chemical Transformation]] | *[[Lidstrom:Chemical Transformation|Chemical Transformation]] | ||
Revision as of 06:51, 20 October 2014
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
- Filtration
Cell Culture:
Methylotrophs
E. coli
OD measurements
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Mutagenesis
Misc.
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
- NEB cloning guide
Protein
Purification
Analytics
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
- NanoDrop for proteins
Chemicals
- Chemical Stability
- Also, see Solution Stock Info
Buffers
E. coli
- E. coli Vector Compatibility (In Progress)
- origins of replication
- Competent Cell Preparation (in progress)
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
- Enzyme Assay Basics
- Enzyme Assay Data Analysis
- Solution Stock Info
- Path Length in 96-well plates
- Reducing Agents
Equipment
- Plate readers:
- Cuvette spectrophotometer:
- Centrifuge:
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
- Flow Cytometry:
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
