Lidstrom:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Equipment)
(Cell Culture:)
(3 intermediate revisions not shown.)
Line 15: Line 15:
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
-
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
+
 
=== Methylotrophs ===
=== Methylotrophs ===
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
Line 22: Line 22:
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
 +
 +
=== OD measurements ===
 +
* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]]
 +
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
==DNA/RNA:==
==DNA/RNA:==
Line 106: Line 110:
**[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
**[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
* Cuvette spectrophotometer:  
* Cuvette spectrophotometer:  
-
** [[Lidstrom:Beckman DU 640B spectrophotometer|Beckman DU 640B spectrophotometer]]
 
** [[Lidstrom:UV-2401PC UV-Vis recording spectrophotometer|UV-2401PC UV-Vis recording spectrophotometer]]
** [[Lidstrom:UV-2401PC UV-Vis recording spectrophotometer|UV-2401PC UV-Vis recording spectrophotometer]]
* Centrifuge:
* Centrifuge:

Revision as of 20:23, 25 March 2014

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project

Personal tools