Lidstrom:Protocols

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(Misc.)
(Cell Culture:)
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*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
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*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
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=== Methylotrophs ===
=== Methylotrophs ===
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
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*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
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=== OD measurements ===
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* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]]
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*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
==DNA/RNA:==
==DNA/RNA:==
===DNA===
===DNA===
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== PCR ==  
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==== PCR ====  
*[[Lidstrom:Colony PCR| Colony PCR]]
*[[Lidstrom:Colony PCR| Colony PCR]]
*[[Lidstrom:PCR| PCR (in progress)]]
*[[Lidstrom:PCR| PCR (in progress)]]
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*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]  
*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]  
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
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*[[Lidstrom:NanoDrop#NanoDrop_for_Proteins|NanoDrop for proteins]]
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== Chemicals ==
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*[[Lidstrom:Chemical Stability|Chemical Stability]]
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* Also, see [[Lidstrom:Solution Stock Info|Solution Stock Info]]
==Buffers==
==Buffers==
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
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==== Misc ====
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*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
== Metabolomics ==
== Metabolomics ==

Revision as of 20:23, 25 March 2014

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project

Personal tools