Lidstrom:Protocols

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(Cell Culture:)
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*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
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*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
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=== Methylotrophs ===
=== Methylotrophs ===
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
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*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
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 +
=== OD measurements ===
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* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]]
 +
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
==DNA/RNA:==
==DNA/RNA:==
===DNA===
===DNA===
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== PCR ==  
+
==== PCR ====  
*[[Lidstrom:Colony PCR| Colony PCR]]
*[[Lidstrom:Colony PCR| Colony PCR]]
*[[Lidstrom:PCR| PCR (in progress)]]
*[[Lidstrom:PCR| PCR (in progress)]]
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*[[Lidstrom:Oligo Orders | Oligo Orders]]
*[[Lidstrom:Oligo Orders | Oligo Orders]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
 +
*[https://www.neb.com/~/media/NebUs/Files/Brochures/Cloning_Guide_1113.pdf NEB cloning guide]
==Protein==
==Protein==
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*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]  
*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]  
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
 +
*[[Lidstrom:NanoDrop#NanoDrop_for_Proteins|NanoDrop for proteins]]
 +
 +
== Chemicals ==
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*[[Lidstrom:Chemical Stability|Chemical Stability]]
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* Also, see [[Lidstrom:Solution Stock Info|Solution Stock Info]]
==Buffers==
==Buffers==
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=== Equipment ===
=== Equipment ===
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*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
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*Plate readers:
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*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
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**[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
-
 
+
**[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
-
*[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]]  goes faster than the regular centrifuges that allow 50mL tubes
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* Cuvette spectrophotometer:
-
 
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** [[Lidstrom:UV-2401PC UV-Vis recording spectrophotometer|UV-2401PC UV-Vis recording spectrophotometer]]
-
*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
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* Centrifuge:
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**[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]]  goes faster than the regular centrifuges that allow 50mL tubes
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* Flow Cytometry:
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**[[Lidstrom: Flow Cytometer| Flow Cytometer]]
===in vitro pathway assays (CO<sub>2</sub> project)===
===in vitro pathway assays (CO<sub>2</sub> project)===
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
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==== Misc ====
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*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
== Metabolomics ==
== Metabolomics ==

Revision as of 20:23, 25 March 2014

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project

Personal tools