Lidstrom:Protocols

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(E. coli)
(Assays)
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==Protein==
==Protein==
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
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=== Protein Concentration Determination ===
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*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
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===General===
===General===
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*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
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=== Equipment ===
*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
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*[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]]  goes faster than the regular centrifuges that allow 50mL tubes
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*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
*[[Lidstrom: Flow Cytometer| Flow Cytometer]]

Revision as of 18:40, 31 October 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Protein Concentration Determination

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools