Lidstrom:Protocols

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(DNA/RNA:)
(Assays)
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*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
=== Methylotrophs ===
=== Methylotrophs ===
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
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==Protein==
==Protein==
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
 +
 +
=== Protein Concentration Determination ===
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*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
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*[[Lidstrom:Buffers|Buffers]]
*[[Lidstrom:Buffers|Buffers]]
-
==E. Coli==
+
==E. coli==
-
*[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility  (In Progress)]]
+
*[[Lidstrom:E. Coli Vector Compatibility| E. coli Vector Compatibility  (In Progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
 +
=== E. coli transformation ===
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
*[[Lidstrom:Electroporation|Electroporation]]
*[[Lidstrom:Electroporation|Electroporation]]
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*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
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==Enzyme Assays==
+
==Assays==
===General===
===General===
-
*[[Lidstrom: Plate Reader| Plate Reader Setup]]
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*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
 +
 
 +
=== Equipment ===
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*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
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*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
 +
 
 +
*[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]]  goes faster than the regular centrifuges that allow 50mL tubes
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*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
===in vitro pathway assays (CO<sub>2</sub> project)===
===in vitro pathway assays (CO<sub>2</sub> project)===

Revision as of 18:40, 31 October 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Protein Concentration Determination

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools