Lidstrom:Protocols

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(Lysing Cells)
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===DNA===
===DNA===
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== PCR ==
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*[[Lidstrom:Colony PCR| Colony PCR]]
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*[[Lidstrom:PCR| PCR (in progress)]]
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*[[Lidstrom:Thermocyclers|Thermocyclers]]
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====Isolation ====
====Isolation ====
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
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====Misc.====
====Misc.====
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*[[Lidstrom:PCR| PCR (in progress)]]
 
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]]
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
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==Protein==
==Protein==
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
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*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
==Buffers==
==Buffers==
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*[[Lidstrom:Competent Cells|Competent Cell Lines]]
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
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== Lysing Cells ==
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== Lysing & Cells & Clarifying Extract ==
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[[Lidstrom:French Press|French Press]]
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*[[Lidstrom:French Press|French Press]]
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[[Lidstrom:French Press|Sonicator]]
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*[[Lidstrom:Sonicator|Sonication]]
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*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
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==Enzyme Assays==
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==Assays==
===General===
===General===
*[[Lidstrom: Plate Reader| Plate Reader Setup]]
*[[Lidstrom: Plate Reader| Plate Reader Setup]]
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*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
===in vitro pathway assays (CO<sub>2</sub> project)===
===in vitro pathway assays (CO<sub>2</sub> project)===

Revision as of 15:57, 28 June 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Buffers

E. Coli

Lysing & Cells & Clarifying Extract

Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

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