Lidstrom:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| (2 intermediate revisions by the same user not shown) | |||
| Line 24: | Line 24: | ||
===DNA=== | ===DNA=== | ||
== PCR == | |||
*[[Lidstrom:Colony PCR| Colony PCR]] | |||
*[[Lidstrom:PCR| PCR (in progress)]] | |||
*[[Lidstrom:Thermocyclers|Thermocyclers]] | |||
====Isolation ==== | ====Isolation ==== | ||
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | *[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | ||
| Line 53: | Line 58: | ||
====Misc.==== | ====Misc.==== | ||
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]] | *[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]] | ||
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | *[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | ||
| Line 62: | Line 66: | ||
==Protein== | ==Protein== | ||
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | *[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | ||
*[[Lidstrom:BCA assay|BCA assay]] | *[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration | ||
==Buffers== | ==Buffers== | ||
| Line 76: | Line 80: | ||
== Lysing & Cells & Clarifying Extract == | == Lysing & Cells & Clarifying Extract == | ||
*[[Lidstrom:French Press|French Press]] | *[[Lidstrom:French Press|French Press]] | ||
*[[Lidstrom:Sonicator| | *[[Lidstrom:Sonicator|Sonication]] | ||
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]] | *[[Lidstrom:Ultracentrifuge|Ultracentrifuge]] | ||
Revision as of 08:04, 13 June 2013
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
Methylotrophs
E. coli
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
Protein
- SDS-PAGE Protein Gels
- BCA assay to determine protein concentration
Buffers
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Lysing & Cells & Clarifying Extract
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
