Lidstrom:Protocols
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==General Lab:== | ==General Lab:== | ||
| + | *[[Lidstrom:Health & Safety|Health & Safety]] | ||
| + | *[[Lidstrom:Finding Chemicals|Finding Chemicals]] | ||
| + | *[[Lidstrom:Dishwasher |Dishwasher]] | ||
*[[Lidstrom:Autoclave |Autoclave]] | *[[Lidstrom:Autoclave |Autoclave]] | ||
*[[Lidstrom:Sterile Technique | Sterile technique]] | *[[Lidstrom:Sterile Technique | Sterile technique]] | ||
*[[Lidstrom:E. Coli' basics| ''E. Coli'' basics]] | *[[Lidstrom:E. Coli' basics| ''E. Coli'' basics]] | ||
*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]] | *[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]] | ||
| + | *[[Lidstrom:Pouring Media Plates|Pouring Media Plates]] | ||
| + | |||
| + | ==Cell Culture:== | ||
| + | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
| + | *[[Lidstrom:M9|M9 recipe]] | ||
| + | *[[Lidstrom:TB|Terrific Broth recipe]] | ||
| + | *[[Lidstrom: Bioscreen|Bioscreen Setup]] | ||
| + | *[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | ||
==DNA/RNA:== | ==DNA/RNA:== | ||
===DNA=== | ===DNA=== | ||
| + | ====Isolation ==== | ||
| + | *[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | ||
| + | *[[Lidstrom:Genomic DNA extraction|Genomic DNA extraction]] | ||
| + | |||
| + | ====Manipulation==== | ||
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]] | *[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]] | ||
| - | |||
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]] | *[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]] | ||
| - | |||
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | *[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | ||
*[[Lidstrom:Ligation |Ligation (in progress)]] | *[[Lidstrom:Ligation |Ligation (in progress)]] | ||
| - | |||
| - | |||
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
| - | *[[ | + | *[[Qiagen_Buffers| Qiagen Buffer Recipes]] |
| - | + | ||
| + | =====Fancy Manipulation===== | ||
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | *[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | ||
| - | *[[Lidstrom: | + | *[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] |
| + | *[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | ||
| + | *[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | ||
| + | |||
| + | ====Obervation==== | ||
| + | *[[Lidstrom:Colony PCR| Colony PCR]] | ||
| + | *[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | ||
| + | *[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | ||
| + | |||
| + | ====Misc.==== | ||
| + | *[[Lidstrom:PCR| PCR (in progress)]] | ||
| + | *[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]] | ||
| + | *[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | ||
| + | *[[Lidstrom:Miniprep |Miniprep (in progress)]] | ||
| + | *[[Lidstrom:Oligo Orders | Oligo Orders]] | ||
| + | *[[Lidstrom:Diluting Primers | Diluting Primers]] | ||
| + | |||
| + | ==Protein== | ||
| + | *[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | ||
| + | |||
| + | ==E. Coli== | ||
| + | *[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility (In Progress)]] | ||
| + | *[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | ||
| + | *[[Lidstrom:Chemical Transformation|Chemical Transformation]] | ||
| + | *[[Lidstrom:Electroporation|Electroporation]] | ||
| + | *[[Lidstrom:Competent Cells|Competent Cell Lines]] | ||
| + | |||
| + | ==Enzyme Assays== | ||
| + | |||
| + | ===General=== | ||
| + | *[[Lidstrom: Plate Reader| Plate Reader Setup]] | ||
| - | === | + | ===in vitro pathway assays (CO<sub>2</sub> project)=== |
| + | *[[Lidstrom:in vitro Pathway Assay: Cell Prep| Cell Prep (in progress)]] | ||
| + | *[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | ||
| + | *[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]] | ||
| - | == | + | == Metabolomics == |
| - | *[[Lidstrom: | + | === CO2 project === |
| + | *[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]] | ||
| + | ** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]] | ||
Revision as of 22:25, 10 February 2013
Back home: Lidstrom
Contents |
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
Isolation
Manipulation
- Intro: Building with DNA (in progress)
- Digestion with Restriction Enzymes
- Gibson Assembly (In progress)
- Ligation (in progress)
- Overlap Extension PCR (in progress)
- Qiagen Buffer Recipes
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
Protein
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
