Lidstrom:Protocols

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(DNA/RNA:)
(Metabolomics)
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*[[Lidstrom:Ligation |Ligation (in progress)]]
*[[Lidstrom:Ligation |Ligation (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 +
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
=====Fancy Manipulation=====
=====Fancy Manipulation=====
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 +
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
====Obervation====
====Obervation====
*[[Lidstrom:Colony PCR| Colony PCR]]
*[[Lidstrom:Colony PCR| Colony PCR]]
 +
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
Line 42: Line 45:
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
-
*[[Lidstrom:Oligo Orders | Oligo Orders (in progress)]]
+
*[[Lidstrom:Oligo Orders | Oligo Orders]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
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==E. Coli==
==E. Coli==
 +
*[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility  (In Progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
-
*[[Lidstrom:Transformation| Transformation (in progress)]]
+
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
 +
*[[Lidstrom:Electroporation|Electroporation]]
 +
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
==Enzyme Assays==
==Enzyme Assays==
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
 +
 +
== Metabolomics ==
 +
=== CO2 project ===
 +
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
 +
** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

Revision as of 15:21, 20 November 2012

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

DNA/RNA:

DNA

Manipulation

Fancy Manipulation

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools