Lidstrom:Protocols: Difference between revisions
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====Obervation==== | ====Obervation==== | ||
*[[Lidstrom:Colony PCR| Colony PCR]] | *[[Lidstrom:Colony PCR| Colony PCR]] | ||
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | |||
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | *[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | ||
Revision as of 06:04, 16 July 2012
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
Manipulation
- Intro: Building with DNA (in progress)
- Digestion with Restriction Enzymes
- Gibson Assembly (In progress)
- Ligation (in progress)
- Overlap Extension PCR (in progress)
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- P1 Phage Transduction (in progress)
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders (in progress)
- Diluting Primers
