Lidstrom:Protocols
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*[[Lidstrom: Plate Reader| Plate Reader Setup]] | *[[Lidstrom: Plate Reader| Plate Reader Setup]] | ||
Revision as of 17:39, 3 July 2012
Back home: Lidstrom
Contents |
General Lab:
- Health & Safety
- Finding Chemicals
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
- Intro: Building with DNA (in progress)
- Colony PCR
- Digestion with Restriction Enzymes
- Diluting Primers
- Gibson Assembly (In progress)
- Ligation (in progress)
- Loading Dye Recipe
- Miniprep (in progress)
- Oligo Orders (in progress)
- Overlap Extension PCR (in progress)
- PCR (in progress)
- Purifying DNA (in progress)
- Sequencing with GeneWiz
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
