Lidstrom:Protocols

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(Misc)
Current revision (09:14, 30 November 2014) (view source)
(Restriction Cloning)
 
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*[[Lidstrom:Autoclave |Autoclave]]
*[[Lidstrom:Autoclave |Autoclave]]
*[[Lidstrom:Sterile Technique | Sterile technique]]
*[[Lidstrom:Sterile Technique | Sterile technique]]
-
*[[Lidstrom:E. Coli' basics| ''E. Coli'' basics]]
+
*[[Lidstrom:E. coli basics|E. coli basics]]
*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]]
*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]]
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]]
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]]
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*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
-
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
+
 
=== Methylotrophs ===
=== Methylotrophs ===
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
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*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
 +
 +
=== OD measurements ===
 +
* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]]
 +
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
==DNA/RNA:==
==DNA/RNA:==
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=====Fancy Manipulation=====
=====Fancy Manipulation=====
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
-
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
+
** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]]
 +
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
==== Restriction Cloning ====
==== Restriction Cloning ====
 +
*[[Lidstrom:Overview of Restriction Enzyme Cloning|Overview of Restriction Enzyme Cloning]]
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
*[[Lidstrom:Ligation |Ligation (in progress)]]
*[[Lidstrom:Ligation |Ligation (in progress)]]
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*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
 +
 +
====Mutagenesis====
 +
*[[Lidstrom:EMS Mutagenesis|EMS Mutagenesis]]
 +
*[[Lidstrom:UV Mutagenesis|UV Mutagenesis]]
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*[[Lidstrom:GeneMorph_II| GeneMorph II]]
====Misc.====
====Misc.====
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==Protein==
==Protein==
 +
=== Purification ===
 +
*[[Lidstrom:His-tag Protein Purification|His-tag Protein Purification]]
 +
 +
=== Analytics ===
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
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==E. coli==
==E. coli==
-
*[[Lidstrom:E. Coli Vector Compatibility| E. coli Vector Compatibility  (In Progress)]]
+
*[[Lidstrom:E. coli Vector Compatibility| E. coli Vector Compatibility  (In Progress)]]
 +
** origins of replication
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
 +
=== E. coli transformation ===
=== E. coli transformation ===
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
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*[[Lidstrom:French Press|French Press]]
*[[Lidstrom:French Press|French Press]]
*[[Lidstrom:Sonicator|Sonication]]
*[[Lidstrom:Sonicator|Sonication]]
 +
*[[Lidstrom:Bug Buster|Bug Buster]]
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
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===General===
===General===
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
 +
*[[Lidstrom:Enzyme Assay Data Analysis|Enzyme Assay Data Analysis]]
*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
 +
*[[Lidstrom:Path Length in microwell plates|Path Length in 96-well plates]]
 +
*[[Lidstrom:Reducing Agents|Reducing Agents]]
=== Equipment ===
=== Equipment ===
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==== Misc ====
==== Misc ====
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
 +
*[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]]
== Metabolomics ==
== Metabolomics ==
 +
*[[Lidstrom:Hot water extraction | Hot water extraction for ''in vivo'' metabolite measurement]]
=== CO2 project ===
=== CO2 project ===
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]
** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

Current revision

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Mutagenesis

Misc.

Protein

Purification

Analytics

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project

Personal tools