Lidstrom:Protocols

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(Assays)
Current revision (14:25, 30 June 2014) (view source)
(Misc)
 
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*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
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*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
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=== Methylotrophs ===
=== Methylotrophs ===
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
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*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
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 +
=== OD measurements ===
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* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]]
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*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
==DNA/RNA:==
==DNA/RNA:==
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=====Fancy Manipulation=====
=====Fancy Manipulation=====
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
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** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]]
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
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*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]]
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 +
====Mutagenesis====
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*[[Lidstrom:EMS Mutagenesis|EMS Mutagenesis]]
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*[[Lidstrom:UV Mutagenesis|UV Mutagenesis]]
====Misc.====
====Misc.====
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==Protein==
==Protein==
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=== Purification ===
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*[[Lidstrom:His-tag Protein Purification|His-tag Protein Purification]]
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=== Analytics ===
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
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===General===
===General===
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
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*[[Lidstrom:Enzyme Assay Data Analysis|Enzyme Assay Data Analysis]]
*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
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*[[Lidstrom:Path Length in microwell plates|Path Length in 96-well plates]]
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*[[Lidstrom:Reducing Agents|Reducing Agents]]
=== Equipment ===
=== Equipment ===
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==== Misc ====
==== Misc ====
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[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
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*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
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*[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]]
== Metabolomics ==
== Metabolomics ==

Current revision

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Mutagenesis

Misc.

Protein

Purification

Analytics

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project

Personal tools