Lidstrom:Preparing Freezer Cell Stocks
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- Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
- Centrifuge for ~2+ minutes & pour off supernatant.
- Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers.
- must use a filter that won't dissolve in DMSO . Most of the filters we have are not appropriate.
- Store at -80oC.
- Test stock after frozen a while.