Lidstrom:Preparing Freezer Cell Stocks

From OpenWetWare

Revision as of 20:29, 10 February 2012 by Janet B. Matsen (Talk | contribs)
Jump to: navigation, search

Back to protocols

DMSO

  • Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
  • Centrifuge for 5-10 minutes & pour off supernatant.
  • Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers.
  • Store at -80oC.
  • Test stock after frozen a while.

Glycerol

Personal tools