Lidstrom:Preparing Freezer Cell Stocks

From OpenWetWare
Revision as of 18:29, 10 February 2012 by Janet B. Matsen (talk | contribs)
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Back to protocols

DMSO

  • Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
  • Centrifuge for 5-10 minutes & pour off supernatant.
  • Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this, I hear it buffers.
  • Store at -80oC.
  • Test stock after frozen a while.

Glycerol

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Lidstrom:Preparing_Freezer_Cell_Stocks&oldid=583172"