Lidstrom:Preparing Freezer Cell Stocks: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 9: | Line 9: | ||
==Glycerol== | ==Glycerol== | ||
* | |||
Revision as of 18:28, 10 February 2012
Back to protocols
DMSO
- Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
- Centrifuge for 5-10 minutes & pour off supernatant.
- Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO.
- Store at -80oC.
- Test stock after frozen a while.
