Lidstrom:Preparing Freezer Cell Stocks

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(New page: Back to protocols Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. Centrifuge for 5-10 minutes & pour off supernatant. Resuspend in 900 uL of HY (hyp...)
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Back to [[Lidstrom: Protocols|protocols]]
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Back to [[Lidstrom:Protocols|protocols]]
Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.

Revision as of 20:27, 10 February 2012

Back to protocols

Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. Centrifuge for 5-10 minutes & pour off supernatant. Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Store at -80oC. Test stock after frozen a while.

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