Lidstrom:PCR: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 2: | Line 2: | ||
==Taq== | ==Taq== | ||
* Cheap | * Cheap | ||
* Use for colony PCR (you don't keep the DNA so higher error rattes are ok) | * Use for colony [[Lidstrom:Colony PCR|PCR]] (you don't keep the DNA so higher error rattes are ok) | ||
==Phusion == | ==Phusion == | ||
* faster, more accurate, & higher processiviy | * faster, more accurate, & higher processiviy | ||
Revision as of 14:05, 3 February 2012
Taq
- Cheap
- Use for colony PCR (you don't keep the DNA so higher error rattes are ok)
Phusion
- faster, more accurate, & higher processiviy
- use for DNA modifications (e.g. things that will be put in vivo)
- more details & instructions: Phusion
- more stable (DNA/polymerase or tRNA/DNA?) interactions at high annealing temps (said Justin Siegal)
- Amanda heard that the Finnzymes version is better than NEB's. I couldn't find supporting information. (Janet)
