Lidstrom:MM1 recipe
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Trace Elements Recipe & Tips
MM1 liquid recipe
- Total volume: 2L (adjust accordingly)
- Solution #1 (1L ddH2O):
- 5.06 g K2HPO4 (Anhydrous)
- 5.18 g NaH2PO4 (Monohydrous)
- Solution #2 (1L ddH2O):
- 0.4 g MgSO4 (Heptahydrous)
- 1 g (NH4)2SO4 (Anhydrous)
- Solution #1 (1L ddH2O):
- Procedure:
- Mix constituents of solution #1 and #2 separately in 2L bottles.
- Currently considering the effects of mixing #1 & #2 before autoclaving.
- Organize tiny bottles to be filled with media post autoclave.
- Each bottle should contain ~150 mL of media.
- Autoclave large bottles and tiny bottles.
- Add 2 mL Visnaic trace elements into bottle #2.
- Pour equal amounts of solution #1 and #2 into the smaller bottles.