Lidstrom:MM1 recipe: Difference between revisions
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(New page: Back to Lidstrom:Protocols == Trace Elements Recipe & Tips == == MM1 liquid recipe == == MM1A recipe ==) |
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== MM1 liquid recipe == | == MM1 liquid recipe == | ||
*Total volume: 2L (adjust accordingly) | |||
**Solution #1 (1L ddH2O): | |||
***5.06 g K2HPO4 (Anhydrous) | |||
***5.18 g NaH2PO4 (Monohydrous) | |||
**Solution #2 (1L ddH2O): | |||
***0.4 g MgSO4 (Heptahydrous) | |||
***1 g (NH4)2SO4 (Anhydrous) | |||
*Procedure: | |||
# Mix constituents of solution #1 and #2 separately in 2L bottles. | |||
##Currently considering the effects of mixing #1 & #2 before autoclaving. | |||
# Organize tiny bottles to be filled with media post autoclave. | |||
##Each bottle should contain ~150 mL of media. | |||
# Autoclave large bottles and tiny bottles. | |||
# Add 2 mL Visnaic trace elements into bottle #2. | |||
# Pour equal amounts of solution #1 and #2 into the smaller bottles. | |||
== MM1A recipe == | == MM1A recipe == | ||
Revision as of 15:15, 5 March 2015
Back to Lidstrom:Protocols
Trace Elements Recipe & Tips
MM1 liquid recipe
- Total volume: 2L (adjust accordingly)
- Solution #1 (1L ddH2O):
- 5.06 g K2HPO4 (Anhydrous)
- 5.18 g NaH2PO4 (Monohydrous)
- Solution #2 (1L ddH2O):
- 0.4 g MgSO4 (Heptahydrous)
- 1 g (NH4)2SO4 (Anhydrous)
- Solution #1 (1L ddH2O):
- Procedure:
- Mix constituents of solution #1 and #2 separately in 2L bottles.
- Currently considering the effects of mixing #1 & #2 before autoclaving.
- Organize tiny bottles to be filled with media post autoclave.
- Each bottle should contain ~150 mL of media.
- Autoclave large bottles and tiny bottles.
- Add 2 mL Visnaic trace elements into bottle #2.
- Pour equal amounts of solution #1 and #2 into the smaller bottles.
