Lidstrom:Gibson Assembly: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Design: - overlap by 20 bp Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not ...) |
No edit summary |
||
| Line 1: | Line 1: | ||
Design: | Design: | ||
- overlap by | - overlap by 40 bp | ||
Misc: | Misc: | ||
Revision as of 07:39, 24 February 2012
Design: - overlap by 40 bp
Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not so much a problem when using chemically competent cells. - Electrocompetence is ~ 1000x more effective so is tempting. -
