Lidstrom:Gene Knockouts: Difference between revisions
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(→pCM184) |
(→pCM184) |
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[[image:pCM157_KOs.png|thumb|upright=4.0|center|Knocking out genes with pCM184_KOs]] | [[image:pCM157_KOs.png|thumb|upright=4.0|center|Knocking out genes with pCM184_KOs]] | ||
Advice: if possible, remove the repetitive sequence between the up/down stream regions and the recombination site recognized by pCM157: causes Sanger sequencing to hiccup. | Not shown: Cre recombinase site, useful for curing antibiotic via pCM157. | ||
Advice: if possible, remove the repetitive sequence (cccccccccccc/gggggggggggg) between the up/down stream regions and the recombination site recognized by pCM157: causes Sanger sequencing to hiccup. | |||
== pCM433KanT== | == pCM433KanT== | ||
Latest revision as of 15:20, 6 June 2016
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pCM184
- original paper: Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Marx (2002)
- sequence: (addgene)
Not shown: Cre recombinase site, useful for curing antibiotic via pCM157.
Advice: if possible, remove the repetitive sequence (cccccccccccc/gggggggggggg) between the up/down stream regions and the recombination site recognized by pCM157: causes Sanger sequencing to hiccup.
pCM433KanT
- original paper: Genetic Tools for the Industrially Promising Methanotroph Methylomicrobium buryatense. Puri (2015)
- sequence
