Lidstrom:Enzyme Assay Basics

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Contents

Resources:

Background

Why rates decline over time

From Enzyme Assays, A Practical Book. By Robert Eisenthal and Michael Danson, 1992. ISBN 0-19-963142-5 or 0-19-963143-3 (paperback). There is also a 2003 edition available @ Amazon & UW libraries.

When you add substrate and enzyme, the rate may be linear for some time then the rate of change may slow. This can be caused by:

  • Substrate depletion
    • You can test whether this is the case by simply adding more substrate.
    • If the initial substrate concentration was much below the Km value, it may be difficult to obtain a prolonged period of linearity unless highly-sensitive assays are used to allow product formation to be detected under conditions where there is a negligible change in substrate concentration.
  • Equilibrium
    • A reversible reaction may be slowing down because it approaches equilibrium. The rate of backward reaction (P -> S) will increase until the rate of product formation and product consumption are equal.
    • You can help this by removing the product as it is formed. A coupled enzyme assay or a reagent that reacts with product may be useful.
  • Product inhibition
    • Products of enzyme-catalyzed reactions are frequently reversible inhibitors of the reaction. Again, use of a system that removes product is helpful.
  • Instability
    • Components of the system may break down, including enzymes and reagents.
    • How to check:
      • Incubate the assay mixture for a series of times under conditions identical to those used in the assay itself but without one of the components (enzyme or substrate(, before starting the reaction by addition of the missing component. If the rates of the reaction are the same whichever component is missing during the pre-incubation period, a loss of linearity due to this cause can probably be excluded.
        • It is important to ensure that the conditions of the pre-incubation are identical to the assay itself. For exmaple, many compounds are light sensitive and this can be a particular problem where relatively high intensities of light are used such as is possible in fluorimetry.
    • Another problem with optical assays is the use of a narrow slit width resulting in localized destruction of only a small portion of the material in the assay cuvette. For example, the fluorescence of tryptophan solutions may decline with time but removal of the cuvette and shaking it can result in an apparent return to the original level of fluorescence if the photo-destruction is limited to only a small proportion of the total protein.
    • It is also possible to for a component in a mixture to appear less' stable under the pre-incubation conditions then when it is in a complete assay mixture. This could result from the substrate being bound by a stabilizing enzyme that protects it from damage from light, etc.
  • Time-dependent inhibition
    • An enzyme might be less stable when catalyzing the reaction than it is under the pre-incubation conditions described in Section 2.1.4. Such an effect would result in a decline in the rate of the reaction with time, whereas the individual components of the assay mixture might appear quite stable during the pre-incubation experiments.
    • To test, you could add more enzyme to the reaction after the reaction ceases. You would expect the rate to be the same as when you started it the first time if the same amount of enzyme is added, unless there has been a significant depletion of substrate(s) or accumulation of inhibitory product(s) during the reaction.
    • The enzyme may also generate an inhibitor that binds the enzyme and prevents it from further catalysis.
      • You can add more enzyme and see if the rate returns.
  • Assay method artefact
    • If the specific detection procedure used ceases to respond linearly to increasing product concentrations this can lead to a decline in the measured rate of the reaction with time.
      • This can happen with spectrophotometry and fluorometry.
    • Coupling enzymes may not respond linearly to product formation over time, for all of the reasons mentioned herein or simply the fact that the coupling system approaching its maximum velocity. Clearly, if such coupled-assay procedures are to be used, it is essential to carry out careful control experiments to prove that the system is capable of providing a true measure of the activity of the enzyme under study at all conditions to be used.
  • Change in assay conditions
    • The rate may change when the reaction conditions change.
    • Example of such a change: pH changes as rxn progresses. H+ ions are generated or consumed during the course of the reaction and it isn't adequately buffered. You drift from the pH optimum. Check the pH before and after a reaction.

Why & how we measure the initial rate

  • Any number of effects can change the velocity of the reaction, so it it best to measure the initial rate, before these factors have their effect.
  • If your initial rate is too fast because of substrate depletion or equilibrium, add less enzyme.
  • People commonly assume that determining the initial rate when <20% of the substrate is consumed is sufficient to ensure linearity, but this is not garunteed for the reasons in the previous section. Also, this is only valid when the initial substrate concentration is in excess of the Km value.
  • You can use polynomial fits to estimate the rate at time = 0.
  • Starting an assay by adding one of the components and ensuring adequate mixing can lead to significant uncertainty about the exact time that the reaction was started.
  • There may be a burst or lag before the true rate of the reaction is established (section 2.4).

Bursts and Lags

With assays that show bursts or lag phases, it is important to determine the cause in order to know which phase of the reaction corresponds to the true 'initial rate' of the reaction. The term "initial rate" is normally used to refer to the steady-state rate of the reaction that is established after any pre-steady-state events have occurred.

Potential reasons:

  • The temperature may change over time. We usually keep reagents and enzyme preparations on ice, but do the assay at elevated temperatures. Thus there is some change in temperature over time.
    • Should we warm up extract/reagent aliquots just before use?
  • Particles may settle as the reaction proceeds, leading to erratic rates. This may be interpreted as a burst or lag phase in the reaction.
    • To test this: mixing the contents of the assay cuvette after the reaction has become linear should result in a second phase of aberrant behavior.
  • Slow detector response
    • A lag phase may occur if the initial response of the detection system is slow.
  • Slow dissociation of a reversible inhibitor or activator
    • Although most reversible enzyme activators and inhibitors will dissociate rapidly from the enzyme when the enzyme-inhibitor mixture is diluted, those that show extremely high affinity for the enzyme may show significant time-dependence in their rates of dissociation from it, and association with it. In such cases dilution of the enzyme-inhibitor mixture into the assay may show a lag as the inhibitor slowly dissociates to its equilibrium value. Conversely, if enzyme is added to a reaction mixture containing inhibitor the rate may slowly decrease until the binding equilibrium has been established.
  • Pre-steady-state transients
    • It may take time for the concentration of the intermediate enzyme-substrate and enzyme-product complexes to rise to their steady-state levels. Usually this is rapid and takes special equipment to detect.
  • Relief of substrate inhibition or activation
    • Many enzymes are inhibited by high concentrations of one or more of their substrates. If the initial substrate concentration added to an assay mixture is sufficient to cause some degree of inhibition, the rate of the reaction will tend to increase with time as substrate utilization decreases the inhibition. Alternatively, an initial burst phase n the progress curve can occur if the substrate also behaves as an activator at higher concentrations. Bursts or lags due to such causes should be eliminated by reducing the initial substrate concentration to a level where inhibition, or activation, does not occur. High-substrate inhibition or activation should, of course, be readily detected by their characteristic effects on the dependence of initial velocity on substrate concentration.
    • A similar lag phase in the reaction progress curve can occur if the substrate solution is contaminated by a small amount of another substrate for the enzyme which has high affinity for it but which is broken down slowly.
  • Activation by product
    • A progress curve that curves upward may be observed if one of the products of the reaction is an activator.
  • Substrate interconversions
    • If a compound exists in more than one form, only one of which is an effective substrate for the enzyme, a slow interconversionb etween these forms can lead to burst or lag phases in the progress curves. Example: sugars in different mutarotated forms.
    • Such effects can also give rise to burst phases if a substrate exists in a slow equilibrium between active and inactive forms where an initial rapid phase, corresponding to the utilization of the active form of the substrate, would be followed by a slower phase determined by the rate of isomerization from inactive to active forms.
    • Also watch out for substrates that can be in polymeric forms.
  • Hysteric effects""
    • It is important to eliminate other possible causes of bursts or lags before attributing unusual shaped curves to this. Example: there are two different forms of the enzyme that have different activities.

Blank rates

  • It is not uncommon to observe an apparent rate of reaction in the absence of one of the components of the complete assay mixture. You must figure ot the cause in order to make appropriate corrections.
    • Try omitting the enzyme and each of the substrates in turn.

Causes:

  • Settling of particles
    • Especially when lysates have organelles.
    • The effect usually slows when particles settle. You can test by re-mixing and see if the rate resumes.
  • Precipitation
    • Gradual precipitation of material in the assay mixture may lead to similar problems as precipitation.
      • You can look for signs of turbidity or visible precipitate formation. You can see if changes in absorbance at wavelengths distant from those where any reaction-dependent changes should occur to monitor changes in turbidity directly.
    • Magnesium or calcium ions are added to many assay mixtures because they are essential for the activity of a number of enzymes. However, if such mixtures contain a strong phosphate buffer then precipitation will occur when the solubility product of calcium or magnesium is exceeded.
    • A more confusing situation can occur if one of the products of the reaction is not very soluble and precipitates during the later stages of the reaction, giving rise to accelerating progress curves. Although the blank rates arising from precipitation directly affect optical assays, such behavior could also invalidate the results obtained with other assay procedures.
  • Contamination of one of the components of the assay mixture
    • The presence of one of the substrates in the enzyme solution can give a blank rate with an incomplete reaction mixture. Crude extracts often have some substrate. If the degree of contamination is quite small, the blank rate from this source would be expected to be non-linear and to cease when the endogenous substrate is exhausted.
      • If the enzyme is quite stable under the assay conditions it may be possible to wait until the blank rate dies away before starting the assay.
      • Alternatively, if the contaminating substrate is a small molecule, it should be possible to remove it by dialysis or gel filtration.
    • Problems from this source would be expected to decrease upon purification of the enzyme.
    • Some commercially available enzymes contain substrate, which has been added for stability; you can remove it with dialysis or gel filtration.
    • It is possible you wouldn't be able to remove contaminants. Examples include gases.
    • You may also cause this issue by cross-contamination, such as putting a used pipette in the wrong reagent tube.
  • Adsorption to assay vessels
    • Proteins may stick to glass. Enzyme can carry over from one experiment to the next. Distilled water may be insufficient to remove the bound enzyme; an acid wash may be required.
    • The use of silicone-treated glass or plastic vessels may minimize this problem but we hav found that not all plastics are inert in this respect. Use disposable cuvettes if suitable for the assay.
  • Non-enzymatic reactions
    • Solutions of NAD(P)H are unstable at pH values below neutrality, leading to a spontaneous fall in absorbance at 340 nm. Blank rates due to non-enzymatic reactions should be subtracted from the rates given in the presence of enzyme.
    • The reaction of exogenous factors can also lead to blank rates. Example: absorption of CO2 in a poorly buffered solution --> lower pH --> blank rate.
    • Example: Aldehydes react with NAD+ to give a product that has similar absorbance to NADH. This reaction can cause significant problems in determining the activity of the aldehyde dehydrogenase at alkaline pH values but is not significant at neutral or acid pHs.
    • reference = Duncan, R. J. S. and Tipton, K. F. (1971). Eur J. Biochem., 22, 257.
  • Contaminating enzymes
    • The presence of another enzyme in the preparation which catalyzes an interfering reaction can give rise to a blank rate. If the substrate for the contaminating enzyme is also a contaminant of the preparation it may be possible to remove it by dialysis or gel filtration. However, this is not always possible. For example, the assay of dehydrogenases in crude tissue preparations may be difficult because of the presence of NADH-cytochrome-c reductase. In this case chytochrome-c is not readily removed by dialysis. In such cases it may be necessary to use an inhibitor of the contaminating enzyme, taking care to ensure that it has no effect on the enzyme under study, or to purify the enzyme in order to remove the contaminating material. It may not be sufficient to use an alternative assay procedure if the other enzyme depletes the substrate.
    • In some cases, contaminating enzymes may require no substrates other than those used by the enzyme under study.

Correction for blank rates

  • It is important to understand the cause of a blank rate before one may make the appropriate corrections for it.
    • In many cases it is possible to obtain the true rate of the enzyme-catalyzed reaction by simply subtracting the blank rate given in a suitable incomplete mixture from that obtained with the full assay.

Controls

  • The basic set of tests you should do:
    • + enzymes + substrate
    • + enzymes - substrate
    • - enzymes + substrate (strain = empty vector control or equivalent)
    • - enzymes - substrate (strain = empty vector control or equivalent)
  • Mary likes to see a plot like
    cartoon of a good way to depict enzyme assay data.  Each bar is Vmax for reaction with substrate - Vmax for rxn without substrate.
    cartoon of a good way to depict enzyme assay data. Each bar is Vmax for reaction with substrate - Vmax for rxn without substrate.
  • You could also subtract the empty vector height from the control height, but Mary said she prefers to see them separately. JM 10/31/2013

Factors to control for

From Wikipedia 2013/11/1

  • Salt Concentration:
    • Most enzymes cannot tolerate extremely high salt concentrations. The ions interfere with the weak ionic bonds of proteins. Typical enzymes are active in salt concentrations of 1-500 mM. As usual there are exceptions such as the halophilic algae and bacteria.
  • Effects of Temperature:
    • All enzymes work within a range of temperature specific to the organism. Increases in temperature generally lead to increases in reaction rates. There is a limit to the increase because higher temperatures lead to a sharp decrease in reaction rates. This is due to the denaturating (alteration) of protein structure resulting from the breakdown of the weak ionic and hydrogen bonding that stabilize the three dimensional structure of the enzyme active site.[11] The "optimum" temperature for human enzymes is usually between 35 and 40 °C. The average temperature for humans is 37 °C. Human enzymes start to denature quickly at temperatures above 40 °C. Enzymes from thermophilic archaea found in the hot springs are stable up to 100 °C.[12] However, the idea of an "optimum" rate of an enzyme reaction is misleading, as the rate observed at any temperature is the product of two rates, the reaction rate and the denaturation rate. If you were to use an assay measuring activity for one second, it would give high activity at high temperatures, however if you were to use an assay measuring product formation over an hour, it would give you low activity at these temperatures.
  • Effects of pH:
    • Most enzymes are sensitive to pH and have specific ranges of activity. All have an optimum pH. The pH can stop enzyme activity by denaturating (altering) the three dimensional shape of the enzyme by breaking ionic, and hydrogen bonds. Most enzymes function between a pH of 6 and 8; however pepsin in the stomach works best at a pH of 2 and trypsin at a pH of 8.
  • Substrate Saturation:
    • Increasing the substrate concentration increases the rate of reaction (enzyme activity). However, enzyme saturation limits reaction rates. An enzyme is saturated when the active sites of all the molecules are occupied most of the time. At the saturation point, the reaction will not speed up, no matter how much additional substrate is added. The graph of the reaction rate will plateau.
  • Level of crowding
    • Large amounts of macromolecules in a solution will alter the rates and equilibrium constants of enzyme reactions, through an effect called macromolecular crowding.[13]

Preparing Samples

Cell Pellet Prep

General guidelines:

  • 50 mL of E. coli in LB/TB is usually plenty. (stationary phase)
  • If using a methylotroph, you may need more culture volume. Some strains are "sick" and don't grow very turbid. Use:
    • 100 mL of at OD 0.6-0.8 ~or~
    • 200 mL at OD = 0.4 ~or~
    • 300 mL at OD = 0.2

Of course the amount of biomass depends on how well your enzyme is expressed and what its specific activity is.

Lysis

  • Use a similar mass of cells for different strains you are breaking.
    • This allows for more accurate BCA results. JM (10/2013) finds strong dependency on protein concentration calculations depending on the dilution used when dilutions span an order of magnitude.
  • Resuspend in 2 mL of an appropriate lysis buffer
  • French press 2-3 times.
  • Centrifuge out debris. Perhaps ultracentrifuge.

Analyze protein concentration

  • 1000 ug/mL is good, says Ceci

Assaying

Assay cells

  • Ceci/Amanda always do 200 uL in assays. There is no reason not to do 100 or 150 uL though. JM (10/2013)
  • Ceci adds 50 uL per rxn. She said you don't want to dilute the enzymes more than you need to, as they are most happy when concentrated.
    • This may require that you use more substrate. Hopefully your substrate is cheap.
    • If you are doing an NADH-linked assay, there is a limit to the amount of NADH you can add, as you will saturate the spectrophotometer.

Validity Check

  • If you reduce the cell extract 2x, do you see Vmax reduce by 2x? (a desired result)
    • Note: crowding does change, so your numbers may not be ideal.
  • If you double the substrate or cofactor concentration(s), does the rate remain unchanged?
    • If the rate changes, it is possible inhibition is occurring. For example, assays that use ATP have ATP degrade to ADP at some rate. If ADP inhibits your assay, then increased in ATP may lead to decreases in Vmax.

Data Processing

Calculating Enzyme Rates

  • The slope provided by the plate reader needs to be converted to moles/time/mg protein or similar units.
  • Convert absorbance to M
    • If using NADH, the extinction coefficient at 340nm is 6220 M-1cm-1
      • If there is less volume in the well, the conversion factor is greater because the path length is larger.
  • Factor in the path length.
    • If using the crystal plate in the plater reader, 200 uL is 0.51 cm. (Elizabeth "Betsy" Skovran figured this out)
  • Normalize for protein concentration.
  • How to convert units from plate reader to mM/min or uM/min:
Sample calculation for converting the milli-units/min provided by the plate reader to concentration change over time.  Calculations assume NADH is being monitored at 340 nm and allows for a flexible number of microliters of total reaction in the well.  Normalizing by protein concentration should be done subsequently.
Sample calculation for converting the milli-units/min provided by the plate reader to concentration change over time. Calculations assume NADH is being monitored at 340 nm and allows for a flexible number of microliters of total reaction in the well. Normalizing by protein concentration should be done subsequently.

Example of Preparing for an assay that monitors NADH consumption

  • First, determine how fast NADH oxidizes in your assay environment. It is pH and buffer dependent. It may not be zero.
  • Determine how fast the reaction proceeds in the absence of your enzymes at various substrate concentrations.
    • Example: look at Vmax as you increase [formate] for an assay that has formate as a substrate and NADH as the cofactor and substance you are monitoring. You should have an increase in Vmax as you increase [formate] because there are formate dehydrogenases present. As you increase [formate] you will saturate these enzymes (curve 1 in the picture below). You may see that increases in [formate] lead to decreases in Vmax (curve 2 in the cartoon below); this is caused by inhibition. You want to chose a value of formate that is high enough to saturate the background FDHs if you want to observe the rate caused by enzymes you are adding.
cartoon of adding increasing [formate] to a strain not expressing the enzymes you are testing for.
cartoon of adding increasing [formate] to a strain not expressing the enzymes you are testing for.

Open Questions

How high can the NADH concentration be before the detector is saturated?

  • Will be dependent on the path length.
  • Can be tested by making a calibration curve with increasing [NADH]
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