Lidstrom:Digestion with Restriction Enzymes: Difference between revisions
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**water to 50 uL (32.5 uL, add first) | **water to 50 uL (32.5 uL, add first) | ||
**If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | **If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | ||
*If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes. | |||
Revision as of 09:09, 12 March 2012
Back to Protocols
- Try it quick and imprecise like iGEM 2011 UW recommends:
- 10 uL DNA
- 5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
- 0.5 uL BSA (if required. See above)
- 1uL enzyme 1
- water to 50 uL (32.5 uL, add first)
- If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
- If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.
