Lidstrom:Digestion with Restriction Enzymes

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**water to 50 uL (32.5 uL, add first)
**water to 50 uL (32.5 uL, add first)
**If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
**If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
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*If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.

Revision as of 12:09, 12 March 2012

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  • If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.
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