Lidstrom:Digestion with Restriction Enzymes: Difference between revisions
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(New page: Back to Protocols *Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends: 10 uL DNA 5uL buffer (check h...) |
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*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends: | *Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends: | ||
10 uL DNA | **10 uL DNA | ||
5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers) | **5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers) | ||
0.5 uL BSA (if required. See above) | **0.5 uL BSA (if required. See above) | ||
1uL enzyme 1 | **1uL enzyme 1 | ||
water to 50 uL (32.5 uL, add first) | **water to 50 uL (32.5 uL, add first) | ||
If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | **If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | ||
Revision as of 13:02, 6 March 2012
Back to Protocols
- Try it quick and imprecise like iGEM 2011 UW recommends:
- 10 uL DNA
- 5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
- 0.5 uL BSA (if required. See above)
- 1uL enzyme 1
- water to 50 uL (32.5 uL, add first)
- If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
